Wholesale Nisin box packaging, targeting Gram-positive bacteria

Nisin is a natural bioactive antibacterial peptide that can effectively kill or inhibit Gram-positive bacteria causing food spoilage.

I. Antibacterial Spectrum of Nisin

Nisin has an inhibitory effect on a variety of Gram-positive bacteria, including Lactobacillus, Clostridium botulinum, Staphylococcus, Listeria, heat-resistant spoilage bacteria, Corynebacterium, Micrococcus, Leuconostoc, Mycobacterium, etc. In particular, it has a strong inhibitory effect on spore-producing bacteria, such as Bacillus, Clostridium, Bacillus thermophilus, Clostridium botulinum, etc. Under specific conditions, such as relatively acidic conditions, Nisin can also kill Gram-negative bacteria and other bacteria.

II. Action Targets of Nisin on Gram-positive Bacteria

Cell Membrane

The "pore theory" suggests that Nisin is adsorbed onto the bacterial cell membrane through electrostatic interactions. The C-terminal and N-terminal of its amino acids bind to the phosphate ester layer on the cell membrane, and then it invades the bacterial cell membrane, forming permeable pores. This changes the osmotic pressure inside and outside the bacterial cell membrane, and the cell dies due to autolysis.

Substances Related to Cell Wall Synthesis

Nisin can bind to lipid II, a precursor substance for the synthesis of the cell wall of Gram-positive bacteria, to form a complex, thus affecting the synthesis process of the cell wall and inhibiting the growth and reproduction of bacteria. For example, Nisin specifically forms a complex with the lipid-II cell wall precursor, thereby affecting the cell wall synthesis process and using it as a docking molecule to form pores. Eventually, this leads to the leakage of cell contents and directly causes cell death.