Nisin in Dairy Product Preservation.


Nisin is a ribosomally synthesized antimicrobial peptide composed of 34 amino acids. It is classified as a bacteriocin, a type of antimicrobial peptide produced by bacteria to inhibit the growth of closely related or competing microorganisms. Nisin's primary source is the lactic acid bacterium Lactococcus lactis, specifically strains belonging to the subspecies Lactococcus lactis subsp. lactis and Lactococcus lactis subsp. cremoris.

Mechanism of Antimicrobial Action

Nisin exerts its antimicrobial effects through several mechanisms, making it a powerful preservative in the dairy industry. The primary modes of action include:

Disruption of Cell Membranes: Nisin has a high affinity for bacterial cell membranes, where it binds to lipid II, an essential precursor in cell wall synthesis. This binding disrupts cell membrane integrity, leading to the leakage of cellular contents and ultimately cell death.

Pore Formation: Nisin can also form pores in the cytoplasmic membrane of susceptible bacteria. These pores disrupt ion gradients and nutrient uptake, further inhibiting bacterial growth.

Inhibition of Spore Outgrowth: Nisin has been shown to inhibit the outgrowth of spore-forming bacteria such as Clostridium and Bacillus species, which are of concern in the dairy industry due to their heat-resistant spores.

Regulatory Status of Nisin

Nisin is considered a safe food preservative by many regulatory authorities worldwide. It is generally recognized as safe (GRAS) by the U.S. Food and Drug Administration (FDA) and has been approved for use in various countries, including the European Union, Canada, and Australia. The Joint Food and Agriculture Organization (FAO)/World Health Organization (WHO) Expert Committee on Food Additives (JECFA) has established an acceptable daily intake (ADI) of nisin as an antimicrobial food additive.

Applications of Nisin in Dairy Product Preservation

Nisin has found numerous applications in the dairy industry for preserving a wide range of products. Some of the most common applications include:

Cheese: Cheese production involves the growth of lactic acid bacteria to ferment milk and transform it into cheese. While these bacteria play a vital role in cheese flavor and texture development, they can also be susceptible to contamination by spoilage and pathogenic bacteria. Nisin can be added during cheese production to inhibit unwanted bacterial growth, extending the shelf life and improving the safety of the final product.

Yogurt: Yogurt is a fermented dairy product produced by the action of lactic acid bacteria. Nisin can be used to prevent the growth of spoilage bacteria and pathogens during yogurt fermentation and subsequent storage, enhancing the product's shelf life and safety.

Milk: In fluid milk and milk-based beverages, nisin can be added to inhibit the growth of spoilage microorganisms and pathogenic bacteria, such as Salmonella and Escherichia coli. This helps maintain the freshness and safety of milk products.

Butter: Butter is a dairy product with a high fat content, making it susceptible to oxidative rancidity and spoilage by microorganisms. Nisin can be incorporated into butter formulations to extend its shelf life by inhibiting microbial growth and lipid oxidation.

Cream and Cream-Based Products: Cream and cream-based products are often used in various culinary applications. Nisin can be added to cream to prevent the growth of spoilage microorganisms and maintain product quality during storage.

Whey-Based Products: Whey, a byproduct of cheese production, is used in the manufacturing of various dairy products, including protein-rich supplements and nutritional beverages. Nisin can be employed to inhibit the growth of microorganisms in whey-based products, extending their shelf life.

Ice Cream: Ice cream is a popular dairy product that can be susceptible to contamination by spoilage bacteria and pathogens. Nisin can be used in ice cream production to enhance its safety and shelf life.

Safety Considerations

The safety of nisin as a food preservative is well-established. It has a long history of use in the dairy industry and has been extensively studied for its toxicological properties. The low molecular weight of nisin ensures that it is rapidly degraded in the human digestive system, with no accumulation in the body. Nisin has a minimal impact on the overall flavor, texture, and nutritional quality of dairy products when used at recommended levels.

However, it is essential to adhere to regulatory guidelines and use nisin at approved concentrations to ensure its safety. Excessive use of nisin can lead to unintended alterations in product characteristics.

Challenges and Future Prospects

While nisin offers significant benefits in dairy product preservation, several challenges and future prospects should be considered:

Regulatory Harmonization: Harmonizing regulatory standards for nisin across different countries and regions is essential to ensure global trade of dairy products that contain nisin as a preservative.

Cost and Availability: The production of nisin can be cost-prohibitive for some smaller dairy producers. Research and development efforts may lead to more cost-effective production methods, increasing its availability and affordability.

Natural and Clean Label Trends: Consumer demand for clean-label and natural products is on the rise. Nisin's status as a natural preservative aligns with these trends and may provide a competitive advantage for dairy manufacturers.

Research and Innovation: Ongoing research into nisin's applications, including its combination with other natural preservatives, may lead to improved preservation techniques and enhanced dairy product quality.

Sustainability: The environmental impact of nisin production and its use in dairy preservation should be considered. Sustainable practices and eco-friendly production methods may become increasingly important.


Nisin, a natural preservative derived from the lactic acid bacterium Lactococcus lactis, has proven to be a valuable tool in dairy product preservation. Its potent antimicrobial properties, coupled with its regulatory approval and safety record, make it an attractive choice for extending the shelf life and improving the safety of various dairy products, including cheese, yogurt, milk, butter, cream, and more.

As the dairy industry continues to evolve, nisin's role in preserving dairy products is likely to expand. However, addressing regulatory harmonization, cost considerations, and sustainability issues will be crucial to realizing its full potential in the future. Nisin's ability to meet consumer demands for natural and clean-label products positions it as a key player in the preservation of dairy products, ensuring that consumers can continue to enjoy high-quality and safe dairy offerings for years to come.