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Industrialization of nisin


Nisin is an antibacterial substance produced through the fermentation of Lactobacillus strains.It finds wide applications in the food, pharmaceutical, and cosmetic industries.The industrial production involves several key steps:

Cultivation of Strains: High-yield Lactobacillus strains are selected and pre-cultivated.The strains are then inoculated into a culture medium with suitable nutrient components.Culture conditions, such as temperature, pH, and oxygen supply, are adjusted to foster bacterial growth to an appropriate stage (usually 12 to 24 hours).

Fermentation Process: The pre-cultivated strains are inoculated into fermentation vessels containing a suitable fermentation substrate, such as sucrose or lactose.Fermentation conditions, including temperature, pH, stirring speed, and oxygen supply, are controlled to facilitate the growth of Lactobacillus and the production of nisin.The fermentation process typically lasts from several days to weeks.

Isolation and Purification: After fermentation, the broth is separated, and methods like centrifugation are employed to separate bacteria and fermentation waste.The resulting liquid, containing nisin, undergoes further purification through processes such as filtration, concentration, and washing to eliminate impurities and harmful substances, yielding a high-purity nisin.

Drying and Formulation: The purified nisin is subjected to drying processes, commonly using techniques like spray drying or freeze drying.The dried bacteriocin can then be utilized in the formulation of various products.

Strict control measures are imperative throughout the industrial production process, including fermentation conditions and purification processes, to enhance the yield and purity of nisin.     Additionally, hygiene and disinfection practices are crucial during production to prevent bacterial cross-contamination and other issues.

Moreover, the production process for nisin can be adjusted and optimized based on specific requirements to improve production efficiency and product quality.As scientific and technological advancements continue, further innovations and improvements are anticipated in the production technology of nisin.

The industrial production of nisin involves multiple steps, including strain cultivation, fermentation, isolation and purification, and drying.Stringent control of production environments and process parameters is essential to ensure product quality and safety.