Nisin has strict requirements for its storage environment, primarily including the following aspects:

1. Temperature

Nisin should be stored in a dry, cool environment, typically recommended at 4–15°C, to avoid adverse effects on its stability and activity caused by high temperatures. Its antibacterial activity may decrease in high-temperature environments, which could affect its efficacy.

2. Humidity

Nisin needs to be stored in a dry environment to prevent moisture absorption. Excessive humidity may lead to hygroscopicity, thereby affecting its quality and stability.

3. Light Exposure

Nisin should be protected from direct sunlight, as prolonged exposure to light can lead to chemical reactions that reduce its antibacterial activity. Therefore, during storage, it should be kept in a light-protected environment.

4. Avoiding Chemical Reactions

During storage, care should also be taken to prevent nisin from reacting with other substances, as this could decrease its antibacterial activity. This necessitates using storage containers with good sealing properties to prevent air, moisture, or other chemicals from coming into direct contact with nisin.

To ensure the stability and activity of nisin, it should be stored in a dry, cool, and light-protected environment, with good sealing of the storage container. Additionally, the storage environment should be regularly checked to ensure it meets the required storage conditions.